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f goodrum  (Addgene inc)


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    Addgene inc f goodrum
    F Goodrum, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcig3+(pcmv-ires_gfp)/pCIG3+(pCMV-IRES-GFP+version+3)+(Plasmid+%2378264)/pm41264417-300-15-17
    Average 93 stars, based on 33 article reviews
    f goodrum - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: Injured mice received intraperitoneal injections of 5-bromo-2 ′ -deoxyuridine (BrdU, 50 mg/kg; Sigma-Aldrich, B5002) three times every 2 h at 3 dpi. .. Plasmid constructs and lentivirus production Lentiviral vectors pCIG3 98 and pLL3.7 99 were gifts from F. Goodrum (Addgene plasmid #78264) and L. Van Parijs (Addgene plasmid #11795), respectively. .. Lentiviral packaging plasmid psPAX2 (Addgene plasmid #12260) and envelope plasmid pMD2.G (Addgene plasmid #12259) were gifts from D. Trono. pCAG-IRES-EGFP (pCAGIG) was kindly provided by C. L. Cepko and T. Matsuda.

    Expressing:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Plasmid Preparation:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Article Title: An inflammatory T-cell-stromal axis contributes to hematopoietic stem/progenitor cell failure and clonal evolution in human myelodysplastic syndrome
    Article Snippet: The plasmid containing the mutant form of IKK-2 (Flag-IKK-2 S177E S181E; referred hereafter as IKK2SE), which has previously been demonstrated to result in constitutive NFκB activation , was obtained from Addgene. .. The IKK2SE fragment was recloned into the pCIG3 (pCMV-IRES-GFP version 3) plasmid (a gift from Felicia Goodrum (Addgene plasmid # 78264)), resulting in pCIG-IKK2SE. pCIG-IKK2SE and empty pCIG3 (pCIG-EV) were used to transfect HEK-293T cells with psPAX2 and pMD2G to produced lentivirus. ..

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: Injured mice received intraperitoneal injections of 5-bromo-2 ′ -deoxyuridine (BrdU, 50 mg/kg; Sigma-Aldrich, B5002) three times every 2 h at 3 dpi. .. Plasmid constructs and lentivirus production Lentiviral vectors pCIG3 98 and pLL3.7 99 were gifts from F. Goodrum (Addgene plasmid #78264) and L. Van Parijs (Addgene plasmid #11795), respectively. .. Lentiviral packaging plasmid psPAX2 (Addgene plasmid #12260) and envelope plasmid pMD2.G (Addgene plasmid #12259) were gifts from D. Trono. pCAG-IRES-EGFP (pCAGIG) was kindly provided by C. L. Cepko and T. Matsuda.

    Modification:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Generated:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Polymerase Chain Reaction:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Amplification:

    Article Title: Systems and methods for determining a treatment course of action
    Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and piRFP670-N1 (a gift from Vladislav Verkhusha, Addgene #45457) to create N-terminal tagged EVL. ..

    Produced:

    Article Title: An inflammatory T-cell-stromal axis contributes to hematopoietic stem/progenitor cell failure and clonal evolution in human myelodysplastic syndrome
    Article Snippet: The plasmid containing the mutant form of IKK-2 (Flag-IKK-2 S177E S181E; referred hereafter as IKK2SE), which has previously been demonstrated to result in constitutive NFκB activation , was obtained from Addgene. .. The IKK2SE fragment was recloned into the pCIG3 (pCMV-IRES-GFP version 3) plasmid (a gift from Felicia Goodrum (Addgene plasmid # 78264)), resulting in pCIG-IKK2SE. pCIG-IKK2SE and empty pCIG3 (pCIG-EV) were used to transfect HEK-293T cells with psPAX2 and pMD2G to produced lentivirus. ..

    other:

    Article Title: Mitochondrial complex IV remodeling in tumor-associated macrophages amplifies interferon signaling and promotes anti-tumor immunity.
    Article Snippet: Article Mitochondrial complex IV remodeling in tumorassociated macrophages amplifies interferon signaling and promotes anti-tumor immunity Graphical abstract Highlights • Complex IV remodeling via NDUFA4 reduction in TAMs boosts anti-tumor immunity • A co-encoded miRNA and CIV isoform decrease NDUFA4 to expand Cxcl9+ IFN-TAMs • NDUFA4 loss triggers mtDNA release to amplify IFN-TAM gene programs via cGAS-STING • RNA-based targeting of this functional switch boosts tumor immunity and ICB therapy Authors Megan L. Clark, Kamen P. Simeonov, Walter K. Mowel, ..., César de la Fuente-Núñez, Andy J. Minn, Jorge Henao-Mejia Correspondence jhena@pennmedicine.upenn.edu In brief Clark et al. reveal that tumor-derived cues induce a functional switch in tumor- associated macrophages (TAMs) through decreased expression of NDUFA4, a mitochondrial complex IV subunit.. This promotes a shift from pro-tumor Spp1+ TAMs to anti-tumor Cxcl9+ IFN-TAMs via cGAS-STING activation, a process that can be harnessed to enhance anti-tumor immunity and ICB responses.. Clark et al., 2025, Immunity 58, 1–18 July 8, 2025 © 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. https://doi.org/10.1016/j.immuni.2025.06.006 ll

    Article Title: CREB-regulated transcription during glycogen synthesis in astrocytes
    Article Snippet: Lentiviral backbone pCIG3 (pCMV-IRES-GFP) and CMV500 A-CREB were purchased from Addgene (#78264 and #33371) and A-CREB sequences were amplified and subcloned to generate pCIG3-ACREB (See Table for primers).

    Virus:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    shRNA:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Recombinant:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Protease Inhibitor:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Immunohistochemistry:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Imaging:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Staining:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    RNAscope:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Chromatin Immunoprecipitation:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Co-Immunoprecipitation Assay:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    In Situ:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.

    Microarray:

    Article Title: Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains pCIG3 Caviness et al. 98 Addgene #78264 pCIG3-Cre This paper N/A pCIG3-Trib3 This paper N/A pLL3.7 Rubinson et al. 99 Addgene #11795 pLL3.7-Control shRNA This paper N/A pLL3.7-Trib3 shRNA This paper N/A pLL3.7-Sox2 shRNA This paper N/A pLL3.7-Atf4 shRNA This paper N/A pLL3.7-C3ar1 shRNA This paper N/A pLL3.7-Malt1 shRNA This paper N/A Chemicals, peptides, and recombinant proteins Rose Bengal Sigma-Aldrich Cat# 330000 Tamoxifen Sigma-Aldrich Cat# T5648 Tamoxifen Selleck Cat# S1238 Sunflower seed oil Sigma-Aldrich Cat# S5007 BrdU Sigma-Aldrich Cat# B5002 Hoechst 33342 Sigma-Aldrich Cat# B2261 FGF2 PeproTech Cat# 100-18B EGF PeproTech Cat# 315-09 Polyethyleneimine Polysciences Cat# 24765 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Trypsin Sigma-Aldrich Cat# T4549 Hyaluronidase Sigma-Aldrich Cat# H3884 DNase I Roche Cat# 1284932 Cytosine β-D-arabinofuranoside hydrochloride (AraC) Sigma-Aldrich Cat# C6645 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Poly-D-lysine hydrobromide Sigma-Aldrich Cat# P6407 N,N ′ -Disuccinimidyl Glutarate FUJIFILM Wako Pure Chemical Corporation Cat# 041-32823 Formaldehyde Sigma-Aldrich Cat# F8775 Protease Inhibitor Cocktail Sigma-Aldrich Cat# P8340 RNasin Ribonuclease Inhibitor Promega Cat# N2115 Cycloheximide Sigma-Aldrich Cat# 01810 Heparin Sigma-Aldrich Cat# H3393 Critical commercial assays BrdU Immunohistochemistry Kit Abcam Cat# ab125306 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye Thermo Fisher Scientific Cat# C10639 NeuroTrace 530/615 red fluorescent Nissl stain Thermo Fisher Scientific Cat# N21482, RRID:AB_2620170 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics Cat# 322350 RNA-Protein Co-Detection Ancillary kit Advanced Cell Diagnostics Cat# 323180 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9003 Nuclear Complex Co-IP Kit Active Motif Cat# 54001 Duolink In Situ Red Starter Kit Sigma-Aldrich Cat# DUO92101 Deposited data Microarray data This paper GEO: GSE275642 (Continued on next page) 20 Cell Reports 44, 116590, December 23, 2025 .. Aldh1L1-GFP mice (stock no. 011015-UCD; https://www.informatics.jax.org/allele/MGI:3843271) were obtained from the GENSAT project.



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